--- a/devtools/client/inspector/inspector.js
+++ b/devtools/client/inspector/inspector.js
@@ -261,18 +261,19 @@ Inspector.prototype = {
       await onAllPanelsUpdated;
       await this.markup.expandNode(this.selection.nodeFront);
     }
 
     // Setup the toolbar only now because it may depend on the document.
     await this.setupToolbar();
 
     // Show the 3 pane onboarding tooltip only if the inspector is visisble since the
-    // Accessibility panel initializes the Inspector.
-    if (this.show3PaneTooltip && this.toolbox.currentToolId === "inspector") {
+    // Accessibility panel initializes the Inspector and if it is not the browser toolbox.
+    if (this.show3PaneTooltip && !this.target.chrome &&
+        this.toolbox.currentToolId === "inspector") {
       this.threePaneTooltip = new ThreePaneOnboardingTooltip(this.toolbox, this.panelDoc);
     }
 
     // Log the 3 pane inspector setting on inspector open. The question we want to answer
     // is:
     // "What proportion of users use the 3 pane vs 2 pane inspector on inspector open?"
     this.telemetry.keyedScalarAdd(THREE_PANE_ENABLED_SCALAR, this.is3PaneModeEnabled, 1);