--- a/browser/components/originattributes/test/browser/browser.ini
+++ b/browser/components/originattributes/test/browser/browser.ini
@@ -27,16 +27,18 @@ support-files =
   file_thirdPartyChild.sharedworker.js
   file_thirdPartyChild.track.vtt
   file_thirdPartyChild.video.ogv
   file_thirdPartyChild.worker.fetch.html
   file_thirdPartyChild.worker.js
   file_thirdPartyChild.worker.request.html
   file_thirdPartyChild.worker.xhr.html
   file_thirdPartyChild.xhr.html
+  file_windowOpenerRestriction.html
+  file_windowOpenerRestrictionTarget.html
   head.js
   test.js
   test.js^headers^
   test.html
   test2.html
   test2.js
   test2.js^headers^
   test_firstParty.html
@@ -66,8 +68,9 @@ support-files =
 [browser_blobURLIsolation.js]
 [browser_imageCacheIsolation.js]
 [browser_sharedworker.js]
 [browser_httpauth.js]
 [browser_clientAuth.js]
 [browser_cacheAPI.js]
 [browser_permissions.js]
 [browser_sanitize.js]
+[browser_windowOpenerRestriction.js]

new file mode 100644
--- /dev/null
+++ b/browser/components/originattributes/test/browser/browser_windowOpenerRestriction.js
@@ -0,0 +1,98 @@
+/**
+ * Bug 1339336 - A test case for testing pref 'privacy.firstparty.isolate.restrict_opener_access'
+ */
+
+const { classes: Cc, Constructor: CC, interfaces: Ci, utils: Cu } = Components;
+
+const FIRST_PARTY_OPENER = "example.com";
+const FIRST_PARTY_TARGET = "example.org";
+const OPENER_PAGE = "http://" + FIRST_PARTY_OPENER + "/browser/browser/components/" +
+                    "originattributes/test/browser/file_windowOpenerRestriction.html";
+const TARGET_PAGE = "http://" + FIRST_PARTY_TARGET + "/browser/browser/components/" +
+                    "originattributes/test/browser/file_windowOpenerRestrictionTarget.html";
+
+function* testPref(aIsPrefEnabled) {
+  // Use a random key so we don't access it in later tests.
+  let cookieStr = "key" + Math.random().toString() + "=" + Math.random().toString();
+
+  // Open the tab for the opener page.
+  let tab = gBrowser.addTab(OPENER_PAGE);
+
+  // Select this tab and make sure its browser is loaded and focused.
+  gBrowser.selectedTab = tab;
+  tab.ownerGlobal.focus();
+
+  let browser = gBrowser.getBrowserForTab(tab);
+  yield BrowserTestUtils.browserLoaded(browser);
+
+  yield ContentTask.spawn(browser, {cookieStr,
+                                    page: TARGET_PAGE,
+                                    isPrefEnabled: aIsPrefEnabled}, function* (obj) {
+    // Acquire the iframe element.
+    let childFrame = content.document.getElementById("child");
+
+    // Insert a cookie into this iframe.
+    childFrame.contentDocument.cookie = obj.cookieStr;
+
+    // Open the tab here and focus on it.
+    let openedPath = obj.page;
+    if (!obj.isPrefEnabled) {
+      // If the pref is not enabled, we pass the cookie value through the query string
+      // to tell the target page that it should check the cookie value.
+      openedPath += "?" + obj.cookieStr;
+    }
+
+    // Issue the opener page to open the target page and focus on it.
+    this.openedWindow = content.open(openedPath);
+    this.openedWindow.focus();
+  });
+
+  // Wait until the target page is loaded.
+  let targetBrowser = gBrowser.getBrowserForTab(gBrowser.selectedTab);
+  yield BrowserTestUtils.browserLoaded(targetBrowser);
+
+  // The target page will do the check and show the result through its title.
+  is(targetBrowser.contentTitle, "pass", "The behavior of window.opener is correct.");
+
+  // Close Tabs.
+  yield ContentTask.spawn(browser, null, function* () {
+    this.openedWindow.close();
+  });
+  yield BrowserTestUtils.removeTab(tab);
+
+  // Reset cookies
+  Services.cookies.removeAll();
+}
+
+add_task(function* runTests() {
+  let tests = [true, false];
+
+  // First, we test the scenario that the first party isolation is enabled.
+  yield SpecialPowers.pushPrefEnv({"set":
+    [["privacy.firstparty.isolate", true]]
+  });
+
+  for (let enabled of tests) {
+    yield SpecialPowers.pushPrefEnv({"set":
+      [["privacy.firstparty.isolate.restrict_opener_access", enabled]]
+    });
+
+    yield testPref(enabled);
+  }
+
+  // Second, we test the scenario that the first party isolation is disabled.
+  yield SpecialPowers.pushPrefEnv({"set":
+    [["privacy.firstparty.isolate", false]]
+  });
+
+  for (let enabled of tests) {
+    yield SpecialPowers.pushPrefEnv({"set":
+      [["privacy.firstparty.isolate.restrict_opener_access", enabled]]
+    });
+
+    // When first party isolation is disabled, this pref will not affect the behavior of
+    // window.opener. And the correct behavior here is to allow access since the iframe in
+    // the opener page has the same origin with the target page.
+    yield testPref(false);
+  }
+});

new file mode 100644
--- /dev/null
+++ b/browser/components/originattributes/test/browser/file_windowOpenerRestriction.html
@@ -0,0 +1,10 @@
+<!DOCTYPE HTML>
+<html>
+<head>
+  <meta http-equiv="content-type" content="text/html; charset=utf-8">
+  <title>Test page for window.opener accessibility</title>
+</head>
+<body>
+  <iframe id="child" name="child" src="http://example.org/browser/browser/components/originattributes/test/browser/file_firstPartyBasic.html"></iframe>
+</body>
+</html>

new file mode 100644
--- /dev/null
+++ b/browser/components/originattributes/test/browser/file_windowOpenerRestrictionTarget.html
@@ -0,0 +1,33 @@
+<!DOCTYPE HTML>
+<html>
+<head>
+  <meta http-equiv="content-type" content="text/html; charset=utf-8">
+  <title>title not set</title>
+  <script>
+    // If the query string is given, we are expecting the window.opener can be accessed
+    // across different first party domains, so we will match the cookie value.
+    // Otherwise, the access of window.opener should be treated as cross-origin.
+    // Therefore, it should fail at this setting.
+    let openerRestriction = true;
+    let cookieValue;
+    if (window.location.search.length > 0) {
+      cookieValue = window.location.search.substr(1);
+      openerRestriction = false;
+    }
+
+    try {
+      let openerFrame = window.opener.frames["child"];
+      let result = openerFrame.document.cookie === cookieValue;
+      if (result && !openerRestriction) {
+        document.title = "pass";
+      }
+    } catch (e) {
+      if (openerRestriction) {
+        document.title = "pass";
+      }
+    }
+  </script>
+</head>
+<body>
+</body>
+</html>